产品描述
APOE(载脂蛋白E)是一种与脂质代谢和神经系统健康密切相关的蛋白质。它可以帮助人体内的脂质分子进行运输和调节,并参与多种生理过程,如神经元的生长和修复、调节免疫反应等。
APOE基因型的不同组合与多种疾病的发生风险密切相关。例如,APOEε4基因型被认为是阿尔茨海默病的一个重要风险因素,而APOEε2基因型则被认为与心血管疾病的发生风险降低有关。因此,APOE在疾病的预防和治疗中具有重要意义,研究APOE的作用和机制对于了解疾病的发生和治疗具有重要意义。
华美生物所提供的Rat apolipoprotein E (Apo-E) ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测大鼠血清、血浆、组织匀浆、细胞培养上清样本中的APOE,其灵敏度为15.6 ng/mL,检测范围为15.6 ng/mL-1000 ng/mL。
别名
ApoeApolipoprotein E ELISA kit; Apo-E ELISA kit
缩写
APOE
Uniprot No
P02650
灵敏度
15.6 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
92 |
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| Range % |
87-98 |
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| 1:200 |
Average % |
98 |
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| Range % |
93-104 |
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| 1:400 |
Average % |
84 |
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| Range % |
82-88 |
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| 1:800 |
Average % |
90 |
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| Range % |
85-97 |
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回收率
| The recovery of rat Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
85-94 |
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| EDTA plasma (n=4) |
94 |
90-99 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 1000 |
0.067 |
0.065 |
0.066 |
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| 500 |
0.121 |
0.125 |
0.123 |
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| 250 |
0.213 |
0.204 |
0.209 |
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| 125 |
0.386 |
0.355 |
0.371 |
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| 62.5 |
0.604 |
0.590 |
0.597 |
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| 31.2 |
0.811 |
0.858 |
0.835 |
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| 15.6 |
1.109 |
1.116 |
1.113 |
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| 0 |
2.155 |
2.214 |
2.185 |
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货期
3-5 working days