产品描述

别名

ALS9 ELISA Kit; AMYOTROPHIC LATERAL SCLEROSIS ELISA Kit; ANG 1 ELISA Kit; ANG ELISA Kit; ANG I ELISA Kit; ANG1 ELISA Kit; ANGI ELISA Kit; ANGI_HUMAN ELISA Kit; Angiogenin ELISA Kit; Angiogenin ribonuclease RNase A family; 5 ELISA Kit; Epididymis luminal protein 168 ELISA Kit; HEL168 ELISA Kit; MGC22466 ELISA Kit; MGC71966 ELISA Kit; Ribonuclease 5 ELISA Kit; Ribonuclease RNase A Family 5 ELISA Kit; RNase 5 ELISA Kit; RNASE4 ELISA Kit; RNASE5 ELISA Kit

缩写

ANG

Uniprot No

P03950

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ANG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:200		Average %		88
		Range %		84-92
1:400		Average %		94
		Range %		90-98
1:800		Average %		99
		Range %		95-104
1:1600		Average %		102
		Range %		96-108

回收率

The recovery of human ANG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		89		93-96
EDTA plasma (n=4)		100		95-105

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected   1000 1.865 1.830 1.848 1.686   500 1.457 1.446 1.452 1.290   250 1.128 1.082 1.105 0.943   125 0.693 0.708 0.701 0.539   62.5 0.437 0.409 0.423 0.261   31.2 0.260 0.243 0.252 0.090   15.6 0.209 0.201 0.205 0.043   0 0.163 0.160 0.162

货期

3-5 working days