产品描述
APOA5是一种由APOA5基因编码的蛋白质,是一种高密度脂蛋白(HDL)相关蛋白质。它主要通过调节脂质代谢来影响血液中三酰甘油(TG)水平。APOA5与APOC3和APOA4一起,通过协同作用来调节血液中TG的水平。APOA5还与非酯化胆固醇(NEFA)代谢和胰岛素抵抗有关。APOA5缺陷可能会导致高TG水平,这是代谢综合征、脂质代谢异常和心血管疾病的风险因素。因此,APOA5成为了心血管疾病风险评估和治疗的潜在靶点。
华美生物所提供的Rat Apolipoprotein A5(apo-A5)ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测大鼠血清、血浆、组织匀浆样本中的APOA5,其灵敏度为7.8 pg/mL,检测范围为31.2 pg/mL-2000 pg/mL。
别名
Apoa5 ELISA kit; Rap3Apolipoprotein A-V ELISA kit; Apo-AV ELISA kit; ApoA-V ELISA kit; Apolipoprotein A5 ELISA kit; Regeneration-associated protein 3 ELISA kit
缩写
APOA5
Uniprot No
Q9QUH3
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat apo-A5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
87 |
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| Range % |
84-90 |
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| 1:200 |
Average % |
99 |
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| Range % |
94-104 |
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| 1:400 |
Average % |
87 |
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| Range % |
84-90 |
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| 1:800 |
Average % |
100 |
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| Range % |
95-105 |
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回收率
| The recovery of rat apo-A5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
90-95 |
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| EDTA plasma (n=4) |
94 |
90-98 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
1.945 |
1.987 |
1.966 |
1.871 |
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| 10 |
1.503 |
1.568 |
1.536 |
1.441 |
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| 5 |
1.115 |
1.042 |
1.079 |
0.984 |
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| 2.5 |
0.717 |
0.737 |
0.727 |
0.632 |
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| 1.25 |
0.406 |
0.415 |
0.411 |
0.316 |
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| 0.625 |
0.268 |
0.271 |
0.270 |
0.175 |
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| 0.312 |
0.161 |
0.163 |
0.162 |
0.067 |
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| 0 |
0.096 |
0.094 |
0.095 |
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货期
3-5 working days