产品描述
CK-MB (肌酸激酶同工酶-MB,Creatine Kinase-MB) 是一种肌酸激酶同工酶,主要分布于心肌细胞中,是一种心肌标志物,用于心肌梗死的诊断。CK-MB可以随着心肌细胞受损而释放到血液中,检测血清中CK-MB的水平可以帮助医生判断心肌梗死的程度。
华美生物所提供的Rat Creatine Kinase MB isoenzyme,CK-MB ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测大鼠血清、尿液、组织匀浆、血浆样本中的CK-MB,其灵敏度为0.078 ng/mL,检测范围为0.312 ng/mL-20 ng/mL。
别名
N/A
缩写
CK-MB
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
100 |
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| Range % |
98-103 |
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| 1:2 |
Average % |
96 |
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| Range % |
92-100 |
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| 1:4 |
Average % |
92 |
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| Range % |
87-98 |
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| 1:8 |
Average % |
89 |
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| Range % |
84-94 |
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回收率
| The recovery of rat CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
100 |
96-104 |
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| EDTA plasma (n=4) |
93 |
89-97 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.646 |
2.687 |
2.667 |
2.564 |
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| 10 |
1.834 |
1.856 |
1.845 |
1.742 |
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| 5 |
1.096 |
1.099 |
1.098 |
0.995 |
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| 2.5 |
0.593 |
0.583 |
0.588 |
0.485 |
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| 1.25 |
0.356 |
0.368 |
0.362 |
0.259 |
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| 0.625 |
0.229 |
0.249 |
0.239 |
0.136 |
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| 0.312 |
0.165 |
0.174 |
0.170 |
0.067 |
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| 0 |
0.101 |
0.105 |
0.103 |
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货期
3-5 working days