产品描述

This Human ACE2 ELISA Kit was designed for the quantitative measurement of Human ACE2 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

别名

ACE 2 ELISA Kit; ACE related carboxypeptidase ELISA Kit; ACE-related carboxypeptidase ELISA Kit; ACE2 ELISA Kit; ACE2_HUMAN ELISA Kit; ACEH ELISA Kit; Angiotensin converting enzyme 2 ELISA Kit; Angiotensin converting enzyme homolog ELISA Kit; Angiotensin converting enzyme like protein ELISA Kit; Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 ELISA Kit; Angiotensin I converting enzyme 2 ELISA Kit; Angiotensin-converting enzyme homolog ELISA Kit; DKFZP434A014 ELISA Kit; EC 3.4.17 ELISA Kit; metalloprotease MPROT 15 ELISA Kit; Metalloprotease MPROT15 ELISA Kit; OTTHUMP00000022963 ELISA Kit; Processed angiotensin-converting enzyme 2 ELISA Kit

缩写

ACE2

Uniprot No

Q9BYF1

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ACE2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		87
		Range %		80-99
1:2		Average %		97
		Range %		90-105
1:4		Average %		95
		Range %		91-107
1:8		Average %		93
		Range %		80-98

回收率

The recovery of human ACE2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		88-98
EDTA plasma (n=4)		97		90-105

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 10 2.216 2.182 2.199 1.955 5 2.012 1.916 1.964 1.720 2.5 1.754 1.643 1.699 1.455 1.25 1.326 1.290 1.308 1.064 0.625 1.008 0.944 0.976 0.732 0.312 0.714 0.676 0.695 0.451 0.156 0.416 0.392 0.404 0.160 0 0.249 0.239 0.244

货期

3-5 working days