产品描述
APOB(Apolipoprotein B)是一种脂蛋白,在人体中主要由肝脏合成,也可以由小肠上皮细胞合成。APOB参与胆固醇和甘油三酯的代谢和运输,是载脂蛋白B100和B48的主要成分。APOB是胆固醇的主要运输载体,通过结合在血浆中的胆固醇和甘油三酯形成不同类型的脂蛋白颗粒来运输这些物质到身体各部位。
APOB与心血管疾病密切相关,过多的APOB会导致胆固醇沉积在血管壁上形成动脉粥样硬化,进而导致心血管疾病的发生。因此,APOB的水平可以作为心血管疾病的诊断和治疗的重要生物标记物和靶点。
华美生物所提供的Human apolipoprotein B100 (Apo-B100) ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测人血清、血浆、细胞培养上清、唾液、尿液样本中的APOB,其灵敏度为19.5 ng/mL,检测范围为78 ng/mL-5000 ng/mL。
别名
Apo B 100 ELISA Kit; Apo B ELISA Kit; Apo B-100 ELISA Kit; Apo B-48 ELISA Kit; Apo B100 ELISA Kit; Apo B48 ELISA Kit; ApoB 100 ELISA Kit; ApoB 48 ELISA Kit; APOB ELISA Kit; APOB_HUMAN ELISA Kit; Apolipoprotein B (including Ag(x) antigen) ELISA Kit; Apolipoprotein B 100 ELISA Kit; Apolipoprotein B 48 ELISA Kit; Apolipoprotein B ELISA Kit; Apolipoprotein B-48 ELISA Kit; Apolipoprotein B100 ELISA Kit; Apolipoprotein B48 ELISA Kit; FLDB ELISA Kit; LDLCQ4 ELISA Kit
缩写
APOB
Uniprot No
P04114
灵敏度
19.5 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human Apo-B100 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:200 |
Average % |
96 |
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| Range % |
92-102 |
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| 1:400 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:800 |
Average % |
104 |
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| Range % |
102-108 |
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| 1:1600 |
Average % |
87 |
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| Range % |
81-92 |
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回收率
| The recovery of human Apo-B100 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
92-102 |
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| EDTA plasma (n=4) |
90 |
87-94 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 5000 |
0.074 |
0.073 |
0.074 |
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| 2500 |
0.118 |
0.121 |
0.120 |
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| 1250 |
0.182 |
0.172 |
0.177 |
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| 625 |
0.272 |
0.284 |
0.278 |
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| 312 |
0.451 |
0.442 |
0.447 |
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| 156 |
0.770 |
0.754 |
0.762 |
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| 78 |
1.122 |
1.054 |
1.088 |
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| 0 |
2.371 |
2.270 |
2.321 |
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货期
3-5 working days