产品描述
APOA1 (Apolipoprotein A1)是一种由肝脏细胞合成的高密度脂蛋白(HDL)的主要结构蛋白质,参与胆固醇的逆向转运和脂质代谢。它与其他蛋白质一起形成HDL颗粒,通过与组织中游离的胆固醇结合并将其运输到肝脏进行代谢,从而降低血液中胆固醇的水平。
华美生物所提供的Human apolipoprotein A1 (Apo-A1) ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、细胞培养上清、唾液、尿液样本中的APOA1,其灵敏度为0.7 ng/mL,检测范围为1.4 ng/mL-1000 ng/mL。
别名
Apo-AI ELISA Kit; ApoA I ELISA Kit; ApoA-I ELISA Kit; APOA1 ELISA Kit; APOA1_HUMAN ELISA Kit; Apolipoprotein A-I(1-242) ELISA Kit; Apolipoprotein A1 ELISA Kit; Apolipoprotein AI ELISA Kit; Apolipoprotein of high density lipoprotein ELISA Kit; ApolipoproteinAI ELISA Kit; Brp14 ELISA Kit; high density lipoprotein uptake ELISA Kit; Ltw1 ELISA Kit; Lvtw1 ELISA Kit; MGC117399 ELISA Kit; Sep1 ELISA Kit; Sep2 ELISA Kit
缩写
APOA1
Uniprot No
P02647
灵敏度
0.7 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:2000 |
Average % |
88 |
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| Range % |
84-92 |
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| 1:4000 |
Average % |
96 |
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| Range % |
92-102 |
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| 1:8000 |
Average % |
95 |
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| Range % |
89-100 |
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| 1:16000 |
Average % |
92 |
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| Range % |
89-96 |
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回收率
| The recovery of human Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
89-98 |
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| EDTA plasma (n=4) |
96 |
91-101 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
1.879 |
1.903 |
1.891 |
1.728 |
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| 500 |
1.592 |
1.612 |
1.602 |
1.439 |
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| 250 |
1.304 |
1.311 |
1.308 |
1.145 |
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| 125 |
0.955 |
0.968 |
0.962 |
0.799 |
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| 62.5 |
0.743 |
0.757 |
0.750 |
0.587 |
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| 31.2 |
0.562 |
0.572 |
0.567 |
0.404 |
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| 15.6 |
0.374 |
0.376 |
0.375 |
0.212 |
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| 0 |
0.161 |
0.165 |
0.163 |
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货期
3-5 working days