产品描述
This Mouse Anti-CCP-Ab ELISA Kit was designed for the quantitative measurement of Mouse Anti-CCP-Ab protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 6.25 U/mL-400 U/mL and the sensitivity is 1.56 U/mL.
缩写
Anti-CCP-Ab
灵敏度
1.56 U/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse anti-CCP-antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
88-101 |
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| 1:2 |
Average % |
97 |
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| Range % |
91-106 |
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| 1:4 |
Average % |
95 |
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| Range % |
90-106 |
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| 1:8 |
Average % |
96 |
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| Range % |
89-104 |
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回收率
| The recovery of mouse anti-CCP-antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
90-110 |
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| EDTA plasma (n=4) |
95 |
83-99 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| U/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.697 |
2.741 |
2.719 |
2.573 |
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| 200 |
2.201 |
2.224 |
2.213 |
2.067 |
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| 100 |
1.507 |
1.483 |
1.495 |
1.349 |
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| 50 |
0.834 |
0.849 |
0.842 |
0.696 |
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| 25 |
0.492 |
0.511 |
0.502 |
0.356 |
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| 12.5 |
0.297 |
0.310 |
0.304 |
0.158 |
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| 6.25 |
0.226 |
0.238 |
0.232 |
0.086 |
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| 0 |
0.148 |
0.144 |
0.146 |
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货期
3-5 working days