产品描述
This Human CCK-8 ELISA Kit was designed for the quantitative measurement of Human CCK-8 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.57 ng/mL-10 ng/mL and the sensitivity is 0.357 ng/mL .
缩写
CCK-8
灵敏度
0.357 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<15% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human CCK-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 85-97 | |
| 1:2 | Average % | 96 | |
| Range % | 90-106 | |
| 1:4 | Average % | 95 | |
| Range % | 86-104 | |
| 1:8 | Average % | 93 | |
| Range % | 88-96 | |
回收率
| The recovery of human CCK-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 98 | 93-108 | |
| EDTA plasma (n=4) | 95 | 89-103 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | | | | 0.57 | 0.048 | 0.051 | 0.050 | | | | 1.14 | 0.162 | 0.153 | 0.158 | | | | 2.29 | 0.376 | 0.363 | 0.370 | | | | 5.71 | 0.838 | 0.842 | 0.840 | | | | 10 | 1.655 | 1.667 | 1.661 | | |
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货期
3-5 working days