产品描述
This Rabbit CK-MB ELISA Kit was designed for the quantitative measurement of Rabbit CK-MB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 mU/mL-100 mU/mL and the sensitivity is 0.39 mU/mL.
缩写
CK-MB
灵敏度
0.39 mU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 89 | |
| Range % | 84-93 | |
| 1:2 | Average % | 95 | |
| Range % | 90-99 | |
| 1:4 | Average % | 94 | |
| Range % | 89-100 | |
| 1:8 | Average % | 102 | |
| Range % | 96-107 | |
回收率
| The recovery of rabbit CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 88 | 83-96 | |
| EDTA plasma (n=4) | 92 | 88-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| mU/ml | OD1 | OD2 | Average | Corrected | | | 100 | 2.889 | 2.984 | 2.937 | 2.833 | | | 50 | 2.435 | 2.566 | 2.501 | 2.397 | | | 25 | 1.815 | 1.923 | 1.869 | 1.765 | | | 12.5 | 1.012 | 1.027 | 1.020 | 0.916 | | | 6.25 | 0.512 | 0.506 | 0.509 | 0.405 | | | 3.12 | 0.324 | 0.311 | 0.318 | 0.214 | | | 1.56 | 0.181 | 0.178 | 0.180 | 0.076 | | | 0 | 0.104 | 0.103 | 0.104 | | |
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货期
3-5 working days