产品描述
This Mouse C-Peptide ELISA Kit was designed for the quantitative measurement of Mouse C-Peptide protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 2.86 ng/mL-50 ng/mL and the sensitivity is 1.786 ng/mL.
缩写
C-Peptide
灵敏度
0.625 ng/ml
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse C-Peptide in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
85-99 |
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| 1:2 |
Average % |
90 |
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| Range % |
85-96 |
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| 1:4 |
Average % |
95 |
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| Range % |
87-106 |
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| 1:8 |
Average % |
95 |
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| Range % |
89-100 |
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回收率
| The recovery of mouse C-Peptide spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
89-100 |
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| EDTA plasma (n=4) |
95 |
91-103 |
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货期
3-5 working days