产品描述

This Human COLEC11 ELISA Kit was designed for the quantitative measurement of Human COLEC11 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 31.2 pg/mL-2000 pg/mL and the sensitivity is 0.039 ng/mL.

别名

CL K1 ELISA Kit; CL K1 I ELISA Kit; CL K1 II ELISA Kit; CL K1 IIa ELISA Kit; CL K1 IIb ELISA Kit; CL-K1 ELISA Kit; CLK1 ELISA Kit; COL11_HUMAN ELISA Kit; COLEC 11 ELISA Kit; COLEC11 ELISA Kit; Collectin 11 ELISA Kit; Collectin kidney I ELISA Kit; Collectin kidney protein 1 ELISA Kit; Collectin sub family member 11 ELISA Kit; Collectin-11 ELISA Kit; Collectin11 ELISA Kit; DKFZp686N1868 ELISA Kit; MGC129470 ELISA Kit; MGC129471 ELISA Kit; MGC3279 ELISA Kit

缩写

COLEC11

Uniprot No

Q9BWP8

灵敏度

0.039 ng/ml.

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human COLEC11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		86
		Range %		80-92
1:2		Average %		99
		Range %		91-110
1:4		Average %		97
		Range %		92-105
1:8		Average %		93
		Range %		90-98

回收率

The recovery of human COLEC11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		96		89-100
EDTA plasma (n=4)		90		86-96

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected   10 2.649 2.462 2.556 2.433   5 2.167 2.054 2.111 1.988   2.5 1.477 1.385 1.431 1.308   1.25 0.821 0.789 0.805 0.682   0.625 0.450 0.432 0.441 0.318   0.312 0.278 0.269 0.274 0.151   0.156 0.207 0.202 0.205 0.082   0 0.124 0.121 0.123

货期

3-5 working days