产品描述
小鼠CD274 ELISA检测试剂盒用于定量检测血清、血浆和组织匀浆中的小鼠CD274蛋白。该ELISA检测试剂盒采用双抗夹心法,检测范围为31.25pg/mL-2000pg/mL,灵敏度为7.81pg/mL。
别名
Cd274 ELISA Kit; B7h1 ELISA Kit; Pdcd1l1 ELISA Kit; Pdcd1lg1 ELISA Kit; Pdl1Programmed cell death 1 ligand 1 ELISA Kit; PD-L1 ELISA Kit; PDCD1 ligand 1 ELISA Kit; Programmed death ligand 1 ELISA Kit; B7 homolog 1 ELISA Kit; B7-H1 ELISA Kit; CD antigen CD274 ELISA Kit
缩写
CD274
Uniprot No
Q9EP73
灵敏度
7.81 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CD274 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
98 |
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| Range % |
92-105 |
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| 1:2 |
Average % |
94 |
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| Range % |
88-97 |
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| 1:4 |
Average % |
85 |
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| Range % |
80-89 |
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| 1:8 |
Average % |
88 |
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| Range % |
83-95 |
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回收率
| The recovery of mouse CD274 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
87-99 |
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| EDTA plasma (n=4) |
99 |
94-104 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.805 |
2.650 |
2.728 |
2.560 |
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| 1000 |
2.404 |
2.375 |
2.390 |
2.222 |
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| 500 |
1.709 |
1.810 |
1.760 |
1.592 |
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| 250 |
0.994 |
1.008 |
1.001 |
0.833 |
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| 125 |
0.595 |
0.547 |
0.571 |
0.403 |
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| 62.5 |
0.372 |
0.355 |
0.364 |
0.196 |
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| 31.25 |
0.257 |
0.255 |
0.256 |
0.088 |
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| 0 |
0.171 |
0.164 |
0.168 |
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货期
3-5 working days