产品描述
胎球蛋白 A,也称为 α-2-HS-糖蛋白 (AHSG),是一种在肝脏中产生并在血液中循环的蛋白质。它在血液凝固和钙稳态等几个生物过程中起着重要作用,还已知对心血管疾病具有保护作用。
华美生物所提供的Human Fetuin A ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、细胞培养上清、组织匀浆、尿液样本中的AHSG,其灵敏度为3.9 ng/mL,检测范围为15.6 ng/mL-1000 ng/mL。
别名
59 kDa bone sialic acid-containing protein ELISA Kit; A2HS ELISA Kit; Aa2-066 ELISA Kit; AHS ELISA Kit; Ahsg alpha-2-HS-glycoprotein ELISA Kit; Ahsg ELISA Kit; Alpha 2 HS Glycoprotein ELISA Kit; Alpha 2 Z globulin ELISA Kit; Alpha-2-HS-glycoprotein ELISA Kit; Alpha-2-HS-glycoprotein chain B ELISA Kit; Alpha-2-Z-globulin ELISA Kit; Asialofetuin ELISA Kit; Ba alpha 2 glycoprotein ELISA Kit; Ba-alpha-2-glycoprotein ELISA Kit; BSP ELISA Kit; Countertrypin ELISA Kit; Fetua ELISA Kit; FETUA_HUMAN ELISA Kit; Fetuin ; mouse; homolog of ELISA Kit; Fetuin A ELISA Kit; Fetuin-A ELISA Kit; Glycoprotein PP63 ELISA Kit; HSGA ELISA Kit; pp63 ELISA Kit
缩写
AHSG
Uniprot No
P02765
灵敏度
3.9 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human fetuin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1000 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:2000 |
Average % |
94 |
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| Range % |
91-99 |
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| 1:4000 |
Average % |
92 |
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| Range % |
88-98 |
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| 1:8000 |
Average % |
93 |
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| Range % |
84-102 |
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回收率
| The recovery of human fetuin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
92-106 |
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| EDTA plasma (n=4) |
98 |
91-105 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.754 |
2.797 |
2.776 |
2.564 |
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| 500 |
2.312 |
2.354 |
2.333 |
2.121 |
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| 250 |
1.563 |
1.554 |
1.559 |
1.347 |
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| 125 |
0.735 |
0.753 |
0.744 |
0.532 |
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| 62.5 |
0.538 |
0.526 |
0.532 |
0.320 |
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| 31.2 |
0.322 |
0.335 |
0.329 |
0.117 |
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| 15.6 |
0.268 |
0.284 |
0.276 |
0.064 |
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| 0 |
0.211 |
0.213 |
0.212 |
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货期
3-5 working days