产品描述
CCR5是一种膜上G蛋白偶联受体,属于趋化因子受体家族。它主要在多种免疫细胞表达,如单核细胞、巨噬细胞和T细胞等。CCR5可以结合多种趋化因子,如CCL5、CCL3和CCL4等,从而引导免疫细胞向受体所在区域移动。除了免疫调节外,CCR5还在病原微生物感染和肿瘤免疫逃逸等过程中发挥重要作用,因此被广泛研究并作为药物治疗的靶点。
华美生物所提供的Human C-C motif chemokine 5 (CCL5/D17S136E/SCYA5) ELISA kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、细胞培养上清、组织匀浆样本中的CCL5,其灵敏度为2.307 pg/mL,检测范围为15.6 pg/mL-1000 pg/mL。
别名
Beta chemokine RANTES ELISA Kit; Beta chemokine RANTES precursor ELISA Kit; C C motif chemokine 5 ELISA Kit; CCL 5 ELISA Kit; CCL5 ELISA Kit; CCL5_HUMAN ELISA Kit; Chemokine (C C motif) ligand 5 ELISA Kit; Chemokine CC Motif Ligand 5 ELISA Kit; D17S136E ELISA Kit; EoCP ELISA Kit; Eosinophil chemotactic cytokine ELISA Kit; MGC17164 ELISA Kit; RANTES(4-68) ELISA Kit; Regulated upon activation normally T expressed and presumably secreted ELISA Kit; SCYA 5 ELISA Kit; SCYA5 ELISA Kit; SIS delta ELISA Kit; SIS-delta ELISA Kit; SISd ELISA Kit; Small inducible cytokine A5 (RANTES) ELISA Kit; Small inducible cytokine A5 ELISA Kit; Small inducible cytokine subfamily A (Cys Cys) member 5 ELISA Kit; Small-inducible cytokine A5 ELISA Kit; T cell specific protein p288 ELISA Kit; T cell specific protein RANTES ELISA Kit; T cell specific RANTES protein ELISA Kit; T cell-specific protein P228 ELISA Kit; T-cell-specific protein RANTES ELISA Kit; TCP 228 ELISA Kit; TCP228 ELISA Kit
缩写
CCL5
Uniprot No
P13501
灵敏度
2.307 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human CCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
92 |
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| Range % |
87-95 |
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| 1:200 |
Average % |
104 |
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| Range % |
96-107 |
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| 1:400 |
Average % |
93 |
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| Range % |
88-97 |
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| 1:800 |
Average % |
99 |
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| Range % |
94-103 |
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回收率
| The recovery of human CCL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
86-98 |
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| EDTA plasma (n=4) |
99 |
92-104 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.671 |
2.542 |
2.607 |
2.511 |
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| 500 |
1.848 |
1.776 |
1.812 |
1.716 |
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| 250 |
1.026 |
1.007 |
1.017 |
0.921 |
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| 125 |
0.575 |
0.583 |
0.579 |
0.483 |
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| 62.5 |
0.332 |
0.344 |
0.338 |
0.242 |
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| 31.2 |
0.258 |
0.247 |
0.253 |
0.157 |
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| 15.6 |
0.160 |
0.172 |
0.166 |
0.070 |
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| 0 |
0.098 |
0.094 |
0.096 |
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货期
3-5 working days