产品描述

This Human CFTR ELISA Kit was designed for the quantitative measurement of Human CFTR protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 28 pg/mL-1800 pg/mL and the sensitivity is 7 pg/mL.

别名

ABC 35 ELISA Kit; ABC35 ELISA Kit; ABCC 7 ELISA Kit; ABCC7 ELISA Kit; ATP binding cassette sub family C member 7 ELISA Kit; ATP Binding Cassette Superfamily C Member 7 ELISA Kit; ATP binding cassette transporter sub family C member 7 ELISA Kit; ATP-binding cassette sub-family C member 7 ELISA Kit; cAMP dependent chloride channel ELISA Kit; cAMP-dependent chloride channel ELISA Kit; CF ELISA Kit; CFTR ELISA Kit; CFTR/MRP ELISA Kit; CFTR_HUMAN ELISA Kit; Channel conductance controlling ATPase ELISA Kit; Channel conductance-controlling ATPase ELISA Kit; Cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub family C; member 7) ELISA Kit; Cystic fibrosis transmembrane conductance regulator ELISA Kit; Cystic fibrosis transmembrane conductance regulator ATP binding cassette sub family C member 7 ELISA Kit; Cystic Fibrosis Transmembrane Regulator ELISA Kit; dJ760C5.1 ELISA Kit; MRP 7 ELISA Kit; MRP7 ELISA Kit; TNR CFTR ELISA Kit

缩写

CFTR

Uniprot No

P13569

灵敏度

7 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CFTR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		87
		Range %		82-93
1:2		Average %		98
		Range %		94-102
1:4		Average %		102
		Range %		98-106
1:8		Average %		95
		Range %		90-100

回收率

The recovery of human CFTR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		91		85-106
EDTA plasma (n=4)		91		88-96

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1800 1.956 2.110 2.033 1.887 900 1.545 1.622 1.584 1.438 450 1.204 1.286 1.245 1.099 225 0.903 0.931 0.917 0.771 112.5 0.531 0.511 0.521 0.375 56 0.337 0.342 0.340 0.194 28 0.248 0.258 0.253 0.107 0 0.141 0.151 0.146

货期

3-5 working days