产品描述

This Human CEACAM8 ELISA Kit was designed for the quantitative measurement of Human CEACAM8 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

别名

Carcinoembryonic antigen CGM6 ELISA Kit; Carcinoembryonic antigen gene family member 6 ELISA Kit; Carcinoembryonic antigen related cell adhesion molecule 8 ELISA Kit; Carcinoembryonic antigen-related cell adhesion molecule 8 ELISA Kit; CD 66b ELISA Kit; CD 67 ELISA Kit; CD66b ELISA Kit; CD66b antigen ELISA Kit; CD67 ELISA Kit; CD67 antigen ELISA Kit; CEACAM 8 ELISA Kit; CEACAM8 ELISA Kit; CEAM8_HUMAN ELISA Kit; CGM 6 ELISA Kit; CGM6 ELISA Kit; NCA 95 ELISA Kit; NCA95 ELISA Kit; Non-specific cross-reacting antigen NCA-95 ELISA Kit; Nonspecific cross reacting antigen NCA 95 ELISA Kit; Nonspecific cross reacting antigen NCA95 ELISA Kit

缩写

CEACAM8

Uniprot No

P31997

灵敏度

0.156 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Others

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CEACAM8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		91
		Range %		85-96
1:2		Average %		96
		Range %		92-103
1:4		Average %		102
		Range %		95-108
1:8		Average %		88
		Range %		86-91

回收率

The recovery of human CEACAM8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		91		86-95
EDTA plasma (n=4)		97		94-101

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 40 2.322 2.525 2.424 2.273 20 1.989 1.966 1.978 1.827 10 1.365 1.481 1.423 1.272 5 0.975 1.052 1.014 0.863 2.5 0.603 0.625 0.614 0.463 1.25 0.369 0.347 0.358 0.207 0.625 0.272 0.280 0.276 0.125 0 0.146 0.155 0.151

货期

3-5 working days