产品描述
CCL2(单核细胞趋化蛋白-2):CCL2是一种趋化因子,可诱导单核细胞、T细胞、B细胞和自然杀伤细胞等免疫细胞的趋向和浸润。它是炎症反应中重要的介质,参与了多种炎性疾病的发生发展。CCL2的生物学含义是调节和介导免疫细胞的迁移和浸润,在感染、自身免疫和肿瘤等多种疾病的病理过程中具有重要作用。
华美生物所提供的Mouse monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP-1/MCAF ELISA kit属于ELISA检测试剂盒,采用双抗夹心法定量检测鼠血清、血浆、组织匀浆样本中的CCL2,其灵敏度为19.5 pg/mL,检测范围为78 pg/mL-5000 pg/mL。
别名
Ccl2 ELISA Kit; Je ELISA Kit; Mcp1 ELISA Kit; Scya2 ELISA Kit; C-C motif chemokine 2 ELISA Kit; Monocyte chemoattractant protein 1 ELISA Kit; Monocyte chemotactic protein 1 ELISA Kit; MCP-1 ELISA Kit; Platelet-derived growth factor-inducible protein JE ELISA Kit; Small-inducible cytokine A2 ELISA Kit
缩写
CCL2
Uniprot No
P10148
灵敏度
19.5 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
82-94 |
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| 1:2 |
Average % |
95 |
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| Range % |
91-98 |
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| 1:4 |
Average % |
102 |
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| Range % |
96-108 |
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| 1:8 |
Average % |
95 |
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| Range % |
89-98 |
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回收率
| The recovery of mouse MCP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
88 |
82-94 |
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| EDTA plasma (n=4) |
97 |
92-104 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 5000 |
1.946 |
1.864 |
1.905 |
1.806 |
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| 2500 |
1.294 |
1.414 |
1.354 |
1.255 |
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| 1250 |
0.861 |
0.843 |
0.852 |
0.753 |
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| 625 |
0.555 |
0.546 |
0.551 |
0.452 |
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| 312 |
0.347 |
0.356 |
0.352 |
0.253 |
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| 156 |
0.267 |
0.258 |
0.263 |
0.164 |
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| 78 |
0.171 |
0.179 |
0.175 |
0.076 |
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| 0 |
0.099 |
0.098 |
0.099 |
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货期
3-5 working days