产品描述

This Human CPS1 ELISA Kit was designed for the quantitative measurement of Human CPS1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

别名

Carbamoyl phosphate synthase [ammonia] ELISA Kit; Carbamoyl phosphate synthase [ammonia] mitochondrial ELISA Kit; Carbamoyl phosphate synthase ELISA Kit; Carbamoyl phosphate synthetase 1 ELISA Kit; Carbamoyl phosphate synthetase 1 mitochondrial ELISA Kit; Carbamoyl phosphate synthetase I ELISA Kit; Carbamoyl-phosphate synthase [ammonia] ELISA Kit; Carbamoyl-phosphate synthetase I ELISA Kit; Carbamoylphosphate synthase ELISA Kit; Carbamoylphosphate synthetase 1 ELISA Kit; Carbamoylphosphate synthetase I ELISA Kit; CPS 1 ELISA Kit; Cps1 ELISA Kit; CPSase 1 ELISA Kit; CPSase I ELISA Kit; CPSASE1 ELISA Kit; CPSM_HUMAN ELISA Kit; mitochondrial ELISA Kit; MS738 ELISA Kit

缩写

CPS1

Uniprot No

P31327

灵敏度

19.5 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CPS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		96
		Range %		90-102
1:2		Average %		100
		Range %		93-107
1:4		Average %		99
		Range %		94-102
1:8		Average %		89
		Range %		83-95

回收率

The recovery of human CPS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		89		85-93
EDTA plasma (n=4)		96		90-102

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected   5000 2.306 2.367 2.337 2.191   2500 2.171 2.191 2.181 2.035   1250 1.807 1.847 1.827 1.681   625 1.393 1.371 1.382 1.236   312.5 0.801 0.824 0.813 0.667   156 0.552 0.591 0.572 0.426   78 0.362 0.371 0.367 0.221   0 0.143 0.149 0.146

货期

3-5 working days