产品描述

This Human COL4A3 ELISA Kit was designed for the quantitative measurement of Human COL4A3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

别名

Alpha 3 type IV collagen ELISA Kit; Alpha3 type IV collagen ELISA Kit; CO4A3_HUMAN ELISA Kit; COL4A 3 ELISA Kit; Col4a3 ELISA Kit; Collagen alpha 3(IV) chain ELISA Kit; Collagen IV alpha 3 polypeptide ELISA Kit; Collagen type IV alpha 3 (Goodpasture antigen) ELISA Kit; Collagen type IV alpha 3 ELISA Kit; Collagen type IV alpha 3 chain ELISA Kit; Goodpasture antigen ELISA Kit; OTTHUMP00000195044 ELISA Kit; Tumstatin ELISA Kit

缩写

COL4A3

Uniprot No

Q01955

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human COL4A3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:5		Average %		104
		Range %		101-107
1:10		Average %		91
		Range %		88-98
1:20		Average %		96
		Range %		91-103
1:40		Average %		89
		Range %		86-92

回收率

The recovery of human COL4A3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		102		99-105
EDTA plasma (n=4)		96		92-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected     10 2.544 2.511 2.528 2.347     5 2.071 2.023 2.047 1.866     2.5 1.356 1.458 1.407 1.226     1.25 0.776 0.774 0.775 0.594     0.625 0.450 0.488 0.469 0.288     0.312 0.292 0.271 0.282 0.101     0.156 0.208 0.220 0.214 0.033     0 0.178 0.184 0.181

货期

3-5 working days