产品描述

This Human AKAP4 ELISA Kit was designed for the quantitative measurement of Human AKAP4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

别名

A kinase (PRKA) anchor protein 4 ELISA Kit; A kinase anchor protein 4 ELISA Kit; A kinase anchor protein 82 kDa ELISA Kit; A-kinase anchor protein 4 ELISA Kit; A-kinase anchor protein 82 kDa ELISA Kit; AKAP 4 ELISA Kit; AKAP 82 ELISA Kit; AKAP-4 ELISA Kit; AKAP4 ELISA Kit; AKAP4_HUMAN ELISA Kit; AKAP82 ELISA Kit; FSC 1 ELISA Kit; FSC1 ELISA Kit; hAKAP 82 ELISA Kit; hAKAP82 ELISA Kit; HI ELISA Kit; Major sperm fibrous sheath protein ELISA Kit; p82 ELISA Kit; PRKA 4 ELISA Kit; PRKA4 ELISA Kit; Protein kinase A anchoring protein 4 ELISA Kit; Protein kinase A-anchoring protein 4 ELISA Kit; Testis specific gene HI ELISA Kit

缩写

AKAP4

Uniprot No

Q5JQC9

灵敏度

6.25 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human AKAP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		94
		Range %		90-99
1:2		Average %		89
		Range %		85-96
1:4		Average %		105
		Range %		100-114
1:8		Average %		85
		Range %		80-89

回收率

The recovery of human AKAP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		98		93-102
EDTA plasma (n=4)		92		88-87

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1600 2.339 2.257 2.298 2.159 800 1.813 1.676 1.745 1.606 400 1.156 1.124 1.140 1.001 200 0.699 0.743 0.721 0.582 100 0.387 0.415 0.401 0.262 50 0.297 0.304 0.301 0.162 25 0.215 0.209 0.212 0.073 0 0.137 0.141 0.139

货期

3-5 working days