产品描述
This Rat cTn-T ELISA Kit was designed for the quantitative measurement of Rat cTn-T protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL .
别名
Tnnt2 ELISA Kit; Troponin T ELISA Kit; cardiac muscle ELISA Kit; TnTc ELISA Kit; Cardiac muscle troponin T ELISA Kit; cTnT ELISA Kit
缩写
cTn-T
Uniprot No
P50753
灵敏度
3.12 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat cTn-T in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 89 | |
| Range % | 82-97 | |
| 1:2 | Average % | 87 | |
| Range % | 81-94 | |
| 1:4 | Average % | 93 | |
| Range % | 87-100 | |
| 1:8 | Average % | 95 | |
| Range % | 89-102 | |
回收率
| The recovery of rat cTn-T spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 90-105 | |
| EDTA plasma (n=4) | 95 | 88-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 800 | 2.187 | 2.102 | 2.145 | 1.986 | | | 400 | 1.731 | 1.641 | 1.686 | 1.527 | | | 200 | 1.254 | 1.204 | 1.229 | 1.070 | | | 100 | 0.926 | 0.879 | 0.903 | 0.744 | | | 50 | 0.593 | 0.554 | 0.574 | 0.415 | | | 25 | 0.302 | 0.279 | 0.291 | 0.132 | | | 12.5 | 0.241 | 0.227 | 0.234 | 0.075 | | | 0 | 0.165 | 0.153 | 0.159 | | |
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货期
3-5 working days