产品描述
This Mouse Aβ1-40 ELISA Kit was designed for the quantitative measurement of Mouse Aβ1-40 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.
别名
amyloid beta peptide 1-40,Aβ1-40,amyloid β peptide 1-40
缩写
Aβ1-40
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Aβ1-40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 89 | |
| Range % | 80-97 | |
| 1:2 | Average % | 95 | |
| Range % | 90-105 | |
| 1:4 | Average % | 95 | |
| Range % | 83-100 | |
| 1:8 | Average % | 93 | |
| Range % | 83-98 | |
回收率
| The recovery of mouse Aβ1-40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 93-106 | |
| EDTA plasma (n=4) | 89 | 86-92 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.637 | 2.739 | 2.688 | 2.512 | | | 1000 | 2.115 | 2.092 | 2.104 | 1.928 | | | 500 | 1.318 | 1.287 | 1.303 | 1.127 | | | 250 | 0.824 | 0.810 | 0.817 | 0.641 | | | 125 | 0.491 | 0.504 | 0.498 | 0.322 | | | 62.5 | 0.322 | 0.315 | 0.319 | 0.143 | | | 31.25 | 0.265 | 0.273 | 0.269 | 0.093 | | | 0 | 0.172 | 0.180 | 0.176 | | |
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货期
3-5 working days