产品描述

This Human CTGF ELISA Kit was designed for the quantitative measurement of Human CTGF protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.75 pg/mL-120 pg/mL and the sensitivity is 1.875 pg/mL.

别名

CCN 2 ELISA Kit; CCN family member 2 ELISA Kit; CCN2 ELISA Kit; Connective tissue growth factor ELISA Kit; Ctgf ELISA Kit; CTGF_HUMAN ELISA Kit; Hcs 24 ELISA Kit; Hcs24 ELISA Kit; Hypertrophic chondrocyte specific protein 24 ELISA Kit; Hypertrophic chondrocyte-specific gene product 24 ELISA Kit; Hypertrophic chondrocyte-specific protein 24 ELISA Kit; IBP-8 ELISA Kit; IGF-binding protein 8 ELISA Kit; IGFBP-8 ELISA Kit; IGFBP8 ELISA Kit; Insulin like Growth Factor Binding Protein 8 ELISA Kit; Insulin-like growth factor-binding protein 8 ELISA Kit; MGC102839 ELISA Kit; NOV 2 ELISA Kit; NOV2 ELISA Kit

缩写

CTGF

Uniprot No

P29279

灵敏度

1.875 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CTGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		97
		Range %		92-103
1:2		Average %		92
		Range %		86-96
1:4		Average %		89
		Range %		84-96
1:8		Average %		86
		Range %		80-90

回收率

The recovery of human CTGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		88		84-90
EDTA plasma (n=4)		92		88-97

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected   120 1.851 1.954 1.903 1.859   60 1.078 1.087 1.083 1.039   26.25 0.513 0.502 0.508 0.464   11.25 0.255 0.268 0.262 0.218   3.75 0.119 0.116 0.118 0.074   0 0.043 0.044 0.044

货期

3-5 working days