产品描述

    CCL2 (C-C Motif Chemokine Ligand 2), 又称单核细胞趋化蛋白-1(MCP-1)，是一种炎症介质，能够促进单核细胞和记忆性T细胞的趋化和浸润。它在多种炎症性疾病中发挥着重要作用，如关节炎、糖尿病、动脉粥样硬化等。CCL2的含量在炎症过程中明显升高，可以被用作炎症状态的指标。

别名

Ccl2 ELISA Kit; Je ELISA Kit; Mcp1 ELISA Kit; Scya2 ELISA Kit; C-C motif chemokine 2 ELISA Kit; Immediate-early serum-responsive protein JE ELISA Kit; Monocyte chemoattractant protein 1 ELISA Kit; Monocyte chemotactic protein 1 ELISA Kit; MCP-1 ELISA Kit; Small-inducible cytokine A2 ELISA Kit

缩写

CCL2

Uniprot No

P14844

灵敏度

7.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of rat MCP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		98
		Range %		91-105
1:2		Average %		95
		Range %		89-98
1:4		Average %		97
		Range %		92-102
1:8		Average %		95
		Range %		86-98

回收率

The recovery of rat MCP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-98
EDTA plasma (n=4)		95		90-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected   2000 2.459 2.445 2.452 2.289   1000 1.924 1.786 1.855 1.692   500 1.262 1.251 1.257 1.094   250 0.767 0.755 0.761 0.598   125 0.473 0.468 0.471 0.308   62.5 0.334 0.327 0.331 0.168   31.25 0.246 0.238 0.242 0.079   0 0.166 0.160 0.163

货期

3-5 working days