产品描述
Col Ⅰ(Ⅰ型胶原蛋白,Type I Collagen)是一种主要存在于动物体内结缔组织中的胶原蛋白。胶原蛋白是组成细胞外基质的重要结构蛋白,对维持组织的稳定性、弹性和机械强度起着关键作用。Col Ⅰ在人体中含量丰富,主要分布于皮肤、骨骼、肌腱、血管壁等组织中。Col Ⅰ的合成和降解过程在组织修复、生长发育和疾病发生中具有重要生物学意义,对于维持正常组织结构和功能具有重要调控作用。
华美生物所提供的Human Collagen Type Ⅰ,Col Ⅰ ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、尿液、细胞培养上清、组织匀浆样本中的Col Ⅰ,其灵敏度为0.39 ng/mL,检测范围为1.56 ng/mL-100 ng/mL。
别名
Collagen Type 1,Col 1,_x000D_Collagen Type I,Col I
缩写
Col Ⅰ
灵敏度
0.39 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
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| Range % |
80-92 |
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| 1:2 |
Average % |
100 |
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| Range % |
91-110 |
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| 1:4 |
Average % |
105 |
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| Range % |
92-110 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-100 |
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回收率
| The recovery of human Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
89-99 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 100 |
2.921 |
2.985 |
2.953 |
2.767 |
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| 50 |
2.356 |
2.378 |
2.367 |
2.181 |
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| 25 |
1.826 |
1.875 |
1.851 |
1.665 |
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| 12.5 |
1.221 |
1.256 |
1.239 |
1.053 |
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| 6.25 |
0.796 |
0.800 |
0.798 |
0.612 |
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| 3.12 |
0.512 |
0.546 |
0.529 |
0.343 |
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| 1.56 |
0.302 |
0.326 |
0.314 |
0.128 |
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| 0 |
0.188 |
0.184 |
0.186 |
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货期
3-5 working days