产品描述

This Human ACTN4 ELISA Kit was designed for the quantitative measurement of Human ACTN4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

别名

actinin 4 ELISA Kit; Actinin alpha 4 ELISA Kit; actinin4 ELISA Kit; ACTN 4 ELISA Kit; ACTN4 ELISA Kit; ACTN4_HUMAN ELISA Kit; Alpha-actinin-4 ELISA Kit; DKFZp686K23158 ELISA Kit; F actin cross linking protein ELISA Kit; F-actin cross-linking protein ELISA Kit; Focal segmental glomerulosclerosis 1 ELISA Kit; FSGS 1 ELISA Kit; FSGS ELISA Kit; FSGS1 ELISA Kit; Non muscle alpha actinin 4 ELISA Kit; Non-muscle alpha-actinin 4 ELISA Kit

缩写

ACTN4

Uniprot No

O43707

灵敏度

7.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ACTN-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:100		Average %		90
		Range %		84-96
1:200		Average %		90
		Range %		84-95
1:400		Average %		99
		Range %		95-105
1:800		Average %		94
		Range %		89-99

回收率

The recovery of human ACTN-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		87-96
EDTA plasma (n=4)		99		90-104

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected   2000 2.256 2.298 2.277 2.130   1000 1.964 1.904 1.934 1.787   500 1.433 1.467 1.450 1.303   250 1.021 1.061 1.041 0.894   125 0.698 0.655 0.677 0.530   62.5 0.478 0.491 0.485 0.338   31.25 0.267 0.283 0.275 0.128   0 0.146 0.148 0.147

货期

3-5 working days