产品描述
This Human CCSA-2 ELISA Kit was designed for the quantitative measurement of Human CCSA-2 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.33 ng/mL-80 ng/mL and the sensitivity is 1.33 ng/mL .
缩写
CCSA-2
灵敏度
1.33 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<10% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<15% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human CCSA-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 95 | |
| Range % | 90-100 | |
| 1:2 | Average % | 92 | |
| Range % | 88-100 | |
| 1:4 | Average % | 98 | |
| Range % | 92-106 | |
| 1:8 | Average % | 95 | |
| Range % | 86-101 | |
回收率
| The recovery of human CCSA-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 89-102 | |
| EDTA plasma (n=4) | 95 | 90-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 80 | 2.015 | 1.970 | 1.993 | 1.946 | | | 40 | 1.344 | 1.358 | 1.351 | 1.304 | | | 20 | 0.799 | 0.801 | 0.800 | 0.753 | | | 6.67 | 0.341 | 0.359 | 0.350 | 0.303 | | | 3.33 | 0.201 | 0.215 | 0.208 | 0.161 | | | 0 | 0.047 | 0.047 | 0.047 | | |
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货期
3-5 working days