产品描述

The Human α-Smooth muscle actin (α-SMA) ELISA Kit can quantitatively detect the content of α-SMA in human serum, urine, or tissue homogenates. This assay employs the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction to achieve the measurement. The solution develops in proportion to the amount of α-SMA in the sample. And the color intensity can be measured at 450 nm through a microplate reader. The kit has undergone rigorous quality validation in many aspects, including sensitivity, specificity, precision, recovery, linearity, and consistency between batches.

Due to its absence in fibroblasts of normal hearts, α-SMA is the definitive marker for the activated myofibroblasts in hypertrophic and fibrotic hearts and heart failure in humans. α-SMA is also considered not only a mediator of traction forces in contractile myofibroblasts, but also a component of the global mechanotransduction system. The study of Arti V.Shinde etc. has shown that α-SMA knockdown attenuates serum-induced contraction of fibroblast-populated collagen pads. And α-SMA overexpression reduces pad contraction, attenuating fibroblast proliferative activity.

别名

a actin ELISA Kit; AAT6 ELISA Kit; ACTA_HUMAN ELISA Kit; ACTA2 ELISA Kit; Actin alpha 2 smooth muscle aorta ELISA Kit; Actin aortic smooth muscle ELISA Kit; Actin; aortic smooth muscle ELISA Kit; ACTSA ELISA Kit; ACTVS ELISA Kit; Alpha 2 actin ELISA Kit; Alpha actin 2 ELISA Kit; Alpha cardiac actin ELISA Kit; alpha sma ELISA Kit; Alpha-actin-2 ELISA Kit; Cell growth inhibiting gene 46 protein ELISA Kit; Cell growth-inhibiting gene 46 protein ELISA Kit; GIG46 ELISA Kit; Growth inhibiting gene 46 ELISA Kit; MYMY5 ELISA Kit

缩写

ACTA2

Uniprot No

P62736

灵敏度

0.78 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human α-SMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		85
		Range %		80-90
1:2		Average %		93
		Range %		91-105
1:4		Average %		94
		Range %		91-110
1:8		Average %		92
		Range %		83-98

回收率

The recovery of human α-SMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		93		87-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 200 2.338 2.193 2.266 2.073 100 1.931 1.893 1.912 1.719 50 1.547 1.464 1.506 1.313 25 1.112 1.069 1.091 0.898 12.5 0.867 0.817 0.842 0.649 6.25 0.524 0.501 0.513 0.320 3.12 0.288 0.271 0.280 0.087 0 0.197 0.188 0.193

货期

3-5 working days