产品描述
This Rat Col Ⅳ ELISA Kit was designed for the quantitative measurement of Rat Col Ⅳ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL .
别名
Collagen Type 4,Col 4,_x000D_
Collagen Type IV,Col IV
缩写
Col Ⅳ
灵敏度
0.39 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat COL Ⅳ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
97 |
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| Range % |
92-105 |
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| 1:2 |
Average % |
96 |
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| Range % |
92-101 |
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| 1:4 |
Average % |
92 |
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| Range % |
87-96 |
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| 1:8 |
Average % |
87 |
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| Range % |
82-94 |
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回收率
| The recovery of rat COL Ⅳ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
90-98 |
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| EDTA plasma (n=4) |
104 |
99-108 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 100 |
2.221 |
2.120 |
2.171 |
2.019 |
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| 50 |
1.441 |
1.340 |
1.391 |
1.239 |
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| 25 |
0.825 |
0.804 |
0.815 |
0.663 |
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| 12.5 |
0.519 |
0.530 |
0.525 |
0.373 |
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| 6.25 |
0.335 |
0.324 |
0.330 |
0.178 |
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| 3.12 |
0.240 |
0.251 |
0.246 |
0.094 |
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| 1.56 |
0.196 |
0.192 |
0.194 |
0.042 |
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| 0 |
0.152 |
0.151 |
0.152 |
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货期
3-5 working days