产品描述

This human GP-IV ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human GP-IV in multiple samples, including serum, plasma, or tissue homogenates. Antibody specific for GP-IV has been pre-coated onto the microplate. Standards and samples are pipetted into the wells and any GP-IV present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated GP-IV antibody is added to the wells. After washing, avidin conjugated HRP is added to the wells, forming an antibody-antigen-enzyme-labeled antibody complex. Following a wash to remove any unbound HRP-avidin, the TMB substrate solution is added to the wells, and the color develops into blue. The color changes from blue to yellow after the addition of stop solution into the wells. The color intensity is in proportion to the amount of GP-IV bound in the initial step.

GP-IV, also called CD36, is a scavenger receptor that plays a role in myocardial tissue uptake of long-chain fatty acids (FAs) and contributes to lipid accumulation and metabolic dysfunction when fat supply is excessive. CD36 is involved in immune modulation, metabolic regulation, and other pathophysiological processes. In renal tubular epithelial cells, CD36 promotes the uptake of AOPPs, resulting in lipotoxicity and renal tubule interstitial fibrosis in diabetic nephropathy. CD36 is related to platelet activation on the platelet. It is decreased in ischemia-reperfusion and pressure overload-caused cardiac hypertrophy but increased in diabetic cardiomyopathy and atherosclerosis.

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These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 160 2.783 2.777 2.780 2.663 80 2.134 2.117 2.126 2.009 40 1.496 1.478 1.487 1.370 20 0.855 0.837 0.846 0.729 10 0.417 0.404 0.411 0.294 5 0.321 0.334 0.328 0.211 2.5 0.225 0.216 0.221 0.104 0 0.118 0.116 0.117

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