产品描述
This Human CKAP4 ELISA Kit was designed for the quantitative measurement of Human CKAP4 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.44 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL.
别名
63 kDa membrane protein ELISA Kit; CKAP 4 ELISA Kit; Ckap4 ELISA Kit; CKAP4_HUMAN ELISA Kit; Cytoskeleton associated protein 4 ELISA Kit; Cytoskeleton-associated protein 4 ELISA Kit; ERGIC 63 ELISA Kit; ERGIC63 ELISA Kit; MGC99554 ELISA Kit; p63 ELISA Kit; Transmembrane protein 63kD endoplasmic reticulum Golgi intermediate compartment ELISA Kit; Type II transmembrane protein p63 ELISA Kit
缩写
CKAP4
Uniprot No
Q07065
灵敏度
5.86 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human CKAP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 86 | | |
| Range % | 83-89 | | |
| 1:2 | Average % | 95 | | |
| Range % | 91-99 | | |
| 1:4 | Average % | 87 | | |
| Range % | 83-90 | | |
| 1:8 | Average % | 99 | | |
| Range % | 92-103 | | |
回收率
| The recovery of human CKAP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 86 | 83-89 | | |
| EDTA plasma (n=4) | 100 | 95-105 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | | 1500 | 2.345 | 2.401 | 2.373 | 2.200 | | | | 750 | 2.013 | 2.112 | 2.063 | 1.890 | | | | 375 | 1.754 | 1.704 | 1.729 | 1.556 | | | | 187.5 | 1.334 | 1.304 | 1.319 | 1.146 | | | | 93.75 | 0.812 | 0.831 | 0.822 | 0.649 | | | | 46.88 | 0.503 | 0.512 | 0.508 | 0.335 | | | | 23.44 | 0.346 | 0.338 | 0.342 | 0.169 | | | | 0 | 0.172 | 0.174 | 0.173 | | | |
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货期
3-5 working days