产品描述

The ELISA Kit is designed to measure human ANXA5 levels in samples, including serum, plasma, cell culture supernates, or cell lysates. It uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of ANXA5 in samples. The color intensity is measured at 450 nm via a microplate reader.

ANXA5 is known to possess anti-inflammatory and anti-apoptotic properties. It also has a role in cytotoxicity. Due to its preferential phosphatidylserine (PS) binding property, ANXA5 has been used as a marker for the detection of apoptosis-undergoing cells. It regulates cell proliferation, signal transduction, and coagulation. In addition, it has been shown that the binding of ANXA5 to PS⁺ apoptotic cells can regulate the PS-mediated immunosuppressive clearance, thus enhancing the immunogenicity of apoptotic cells, including irradiated, apoptotic-tumor cells. Also, overexpression of ANXA5 has been detected in many cancers, including renal cell cancer, hepatocellular carcinoma, colorectal cancer, and breast cancer.

别名

Anchorin CII ELISA Kit; Annexin 5 ELISA Kit; Annexin A5 ELISA Kit; Annexin V ELISA Kit; Annexin-5 ELISA Kit; Annexin5 ELISA Kit; AnnexinA5 ELISA Kit; AnnexinV ELISA Kit; ANX 5 ELISA Kit; ANX A5 ELISA Kit; ANX5 ELISA Kit; ANXA5 ELISA Kit; ANXA5_HUMAN ELISA Kit; Calphobindin I ELISA Kit; CBP-I ELISA Kit; Endonexin II ELISA Kit; ENX 2 ELISA Kit; ENX2 ELISA Kit; Lipocortin V ELISA Kit; PAP I ELISA Kit; PAP-I ELISA Kit; Placental anticoagulant protein 4 ELISA Kit; Placental anticoagulant protein I ELISA Kit; PLACENTAL PROTEIN 4 ELISA Kit; PP 4 ELISA Kit; Pp4 ELISA Kit; RPRGL3 ELISA Kit; Thromboplastin inhibitor ELISA Kit; VAC-alpha ELISA Kit; Vascular anticoagulant-alpha ELISA Kit

缩写

ANXA5

Uniprot No

P08758

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ANX-V in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	107
Range %	96-112
1:2	Average %	101
Range %	91-107
1:4	Average %	97
Range %	86-103
1:8	Average %	103
Range %	95-107

回收率

The recovery of human ANX-V spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		100		90-106
EDTA plasma (n=4)		102		95-107

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected     20 2.722 2.635 2.679 2.556     10 2.081 1.963 2.022 1.899     5 1.455 1.401 1.428 1.305     2.5 0.948 0.917 0.933 0.810     1.25 0.623 0.608 0.616 0.493     0.625 0.339 0.326 0.333 0.210     0.312 0.231 0.229 0.230 0.107     0 0.125 0.121 0.123

货期

3-5 working days