产品描述
This Rat CCP Ab ELISA Kit was designed for the quantitative measurement of Rat CCP Ab protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 3.12 IU/mL--200 IU/mL and the sensitivity is 0.78 IU/mL.
缩写
CCP Ab
灵敏度
0.78 IU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat anti-CCP-antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
96 |
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| Range % |
85-105 |
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| 1:2 |
Average % |
97 |
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| Range % |
84-105 |
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| 1:4 |
Average % |
96 |
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| Range % |
92-100 |
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| 1:8 |
Average % |
95 |
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| Range % |
88-105 |
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回收率
| The recovery of rat anti-CCP-antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
86 |
83-96 |
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| EDTA plasma (n=4) |
94 |
85-105 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| IU/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.614 |
2.712 |
2.663 |
2.550 |
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| 100 |
1.995 |
2.012 |
2.004 |
1.891 |
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| 50 |
1.282 |
1.267 |
1.275 |
1.162 |
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| 25 |
0.682 |
0.699 |
0.691 |
0.578 |
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| 12.5 |
0.414 |
0.422 |
0.418 |
0.305 |
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| 6.25 |
0.257 |
0.256 |
0.257 |
0.144 |
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| 3.12 |
0.202 |
0.192 |
0.197 |
0.084 |
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| 0 |
0.110 |
0.115 |
0.113 |
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货期
3-5 working days