产品描述
The human bactericidal/permeability-increasing protein (BPI) ELISA Kit is used to quantitatively measure human BPI concentrations in serum, plasma, or cell culture supernates. It performs well in important characteristics, including sensitivity and specificity. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of BPI in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.
BPI, a neutrophil-derived protein, is the natural antagonist of LBP since the interaction of BPI with its ligand LPS is strong enough to prohibit the LPS-dependent stimulation of immune cells. In the context of Gram-negative infection, BPI neutralizes the endotoxic activity of lipopolysaccharides, and thus inhibits hyperactivation. BPI functions as an immune-enhancing pattern recognition molecule in Gram-positive infections. In addition to acting as an LPS-neutralizing protein, BPI also has other functions such as inhibition of endothelial cell growth and dendritic cell maturation or being an anti-angiogenic, chemoattractant, or opsonization agent.
别名
Bactericidal permeability-increasing protein ELISA Kit; Bactericidal/permeability increasing protein ELISA Kit; BPI ELISA Kit; BPI fold containing family D; member 1 ELISA Kit; BPI_HUMAN ELISA Kit; BPIFD1 ELISA Kit; CAP 57 ELISA Kit; CAP57 ELISA Kit; rBPI ELISA Kit; Recombinant BPI holoprotein; rBPI ELISA Kit
缩写
BPI
Uniprot No
P17213
灵敏度
0.31 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human BPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 93 | | |
| Range % | 89-97 | | |
| 1:2 | Average % | 102 | | |
| Range % | 98-106 | | |
| 1:4 | Average % | 90 | | |
| Range % | 85-94 | | |
| 1:8 | Average % | 98 | | |
| Range % | 94-102 | | |
回收率
| The recovery of human BPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 89 | 84-93 | | |
| EDTA plasma (n=4) | 92 | 88-96 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | | 80 | 2.695 | 2.732 | 2.714 | 2.618 | | | | 40 | 2.285 | 2.154 | 2.220 | 2.124 | | | | 20 | 1.431 | 1.366 | 1.399 | 1.303 | | | | 10 | 0.827 | 0.881 | 0.854 | 0.758 | | | | 5 | 0.412 | 0.429 | 0.421 | 0.325 | | | | 2.5 | 0.258 | 0.247 | 0.253 | 0.157 | | | | 1.25 | 0.169 | 0.175 | 0.172 | 0.076 | | | | 0 | 0.094 | 0.098 | 0.096 | | | |
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货期
3-5 working days
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