产品描述
This Mouse APOH ELISA Kit was designed for the quantitative measurement of Mouse APOH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL.
别名
Apoh ELISA kit; B2gp1Beta-2-glycoprotein 1 ELISA kit; APC inhibitor ELISA kit; Activated protein C-binding protein ELISA kit; Apolipoprotein H ELISA kit; Apo-H ELISA kit; Beta-2-glycoprotein I ELISA kit; B2GPI ELISA kit; Beta(2)GPI ELISA kit
缩写
APOH
Uniprot No
Q01339
灵敏度
3.9 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Apo-H in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1000 |
Average % |
88 |
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| Range % |
83-92 |
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| 1:2000 |
Average % |
97 |
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| Range % |
91-101 |
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| 1:4000 |
Average % |
89 |
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| Range % |
84-92 |
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| 1:8000 |
Average % |
97 |
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| Range % |
91-101 |
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回收率
| The recovery of mouse Apo-H spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
87-95 |
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| EDTA plasma (n=4) |
103 |
98-105 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.669 |
2.625 |
2.647 |
2.512 |
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| 500 |
2.180 |
2.112 |
2.146 |
2.011 |
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| 250 |
1.611 |
1.600 |
1.606 |
1.471 |
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| 125 |
0.859 |
0.831 |
0.845 |
0.710 |
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| 62.5 |
0.515 |
0.527 |
0.521 |
0.386 |
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| 31.2 |
0.321 |
0.333 |
0.327 |
0.192 |
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| 15.6 |
0.248 |
0.242 |
0.245 |
0.110 |
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| 0 |
0.136 |
0.134 |
0.135 |
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货期
3-5 working days