产品描述
Aβ1-42 (Amyloid beta 1-42)是由脑细胞中酶β-和γ-分泌酶所作用的Amyloid precursor protein (APP)切割而成的42个氨基酸长的肽段。Aβ1-42在早期阶段是神经退行性疾病如阿尔茨海默病的标志物之一。在病变形成的初期,Aβ1-42聚集成神经毒性的β淀粉样物,进而导致神经元死亡和神经退化。因此,Aβ1-42在诊断和研究神经退行性疾病方面具有重要意义。Aβ1-42的含量可以通过脑脊液和血液等生物标本检测。
华美生物所提供的Mouse amyloid beta peptide 1-42,Aβ1-42 ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测鼠血清、血浆、组织匀浆样本中的Aβ1-42,其灵敏度为3.9 pg/mL,检测范围为15.6 pg/mL-1000 pg/mL。
别名
Amyloid beta 42(ABeta 42),amyloid beta peptide 1-42,Aβ1-42,amyloid βpeptide 1-42
缩写
Aβ1-42
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Aβ1-42 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
88-99 |
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| 1:2 |
Average % |
95 |
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| Range % |
85-105 |
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| 1:4 |
Average % |
96 |
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| Range % |
90-105 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-100 |
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回收率
| The recovery of mouse Aβ1-42 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
90-100 |
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| EDTA plasma (n=4) |
93 |
85-96 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.490 |
2.451 |
2.471 |
2.337 |
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| 500 |
2.049 |
1.985 |
2.017 |
1.883 |
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| 250 |
1.357 |
1.385 |
1.371 |
1.237 |
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| 125 |
0.866 |
0.837 |
0.852 |
0.718 |
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| 62.5 |
0.517 |
0.524 |
0.521 |
0.387 |
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| 31.2 |
0.347 |
0.355 |
0.351 |
0.217 |
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| 15.6 |
0.244 |
0.228 |
0.236 |
0.102 |
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| 0 |
0.135 |
0.132 |
0.134 |
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货期
3-5 working days