产品描述
This Rabbit APOA1 ELISA Kit was designed for the quantitative measurement of Rabbit APOA1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 μg/mL-400 μg/mL and the sensitivity is 1.56 μg/mL.
别名
APOA1Apolipoprotein A-I ELISA kit; Apo-AI ELISA kit; ApoA-I ELISA kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA kit
缩写
APOA1
Uniprot No
P09809
灵敏度
1.56 μg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 84 | |
| Range % | 80-92 | |
| 1:2 | Average % | 99 | |
| Range % | 91-105 | |
| 1:4 | Average % | 89 | |
| Range % | 80-96 | |
| 1:8 | Average % | 94 | |
| Range % | 85-99 | |
回收率
| The recovery of rabbit Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 93 | 89-98 | |
| EDTA plasma (n=4) | 98 | 89-107 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| µg/ml | OD1 | OD2 | Average | Corrected | | | 400 | 2.748 | 2.712 | 2.730 | 2.645 | | | 200 | 2.234 | 2.222 | 2.228 | 2.143 | | | 100 | 1.517 | 1.446 | 1.482 | 1.397 | | | 50 | 0.875 | 0.839 | 0.857 | 0.772 | | | 25 | 0.433 | 0.424 | 0.429 | 0.344 | | | 12.5 | 0.212 | 0.206 | 0.209 | 0.124 | | | 6.25 | 0.141 | 0.138 | 0.140 | 0.055 | | | 0 | 0.087 | 0.083 | 0.085 | | |
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货期
3-5 working days