产品描述

This Human CLEC2D ELISA Kit was designed for the quantitative measurement of Human CLEC2D protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

别名

C type lectin related f ELISA Kit; C type lectin superfamily 2; member D ELISA Kit; C-type lectin domain family 2 member D ELISA Kit; CLAX ELISA Kit; CLC2D_HUMAN ELISA Kit; CLEC2D ELISA Kit; CLEC2D protein ELISA Kit; Clec2d5 ELISA Kit; L LT1 ELISA Kit; Lectin like NK cell receptor ELISA Kit; Lectin like transcript 1 ELISA Kit; Lectin-like NK cell receptor ELISA Kit; Lectin-like transcript 1 ELISA Kit; LLT-1 ELISA Kit; LLT1 ELISA Kit; OCIL ELISA Kit; Osteoclast inhibitory lectin ELISA Kit

缩写

CLEC2D

Uniprot No

Q9UHP7

灵敏度

7.81 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CLEC2D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		89
		Range %		85-93
1:2		Average %		95
		Range %		92-99
1:4		Average %		87
		Range %		83-91
1:8		Average %		90
		Range %		84-97

回收率

The recovery of human CLEC2D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		87-97
EDTA plasma (n=4)		96		90-101

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 2000 2.575 2.600 2.588 2.445 1000 2.215 2.147 2.181 2.038 500 1.533 1.498 1.516 1.373 250 0.901 0.944 0.923 0.780 125 0.531 0.565 0.548 0.405 62.5 0.365 0.344 0.355 0.212 31.25 0.258 0.250 0.254 0.111 0 0.139 0.147 0.143

货期

3-5 working days