产品描述
This Rat CSF1 ELISA Kit was designed for the quantitative measurement of Rat CSF1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
Csf1 ELISA kit; CsfmMacrophage colony-stimulating factor 1 ELISA kit; CSF-1 ELISA kit; MCSF) [Cleaved into: Processed macrophage colony-stimulating factor 1] ELISA kit
缩写
CSF1
Uniprot No
Q8JZQ0
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat M-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 88 | |
| Range % | 82-95 | |
| 1:2 | Average % | 98 | |
| Range % | 90-105 | |
| 1:4 | Average % | 95 | |
| Range % | 90-100 | |
| 1:8 | Average % | 90 | |
| Range % | 85-95 | |
回收率
| The recovery of rat M-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 98 | 92-104 | |
| EDTA plasma (n=4) | 92 | 87-97 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.413 | 2.352 | 2.383 | 2.192 | | | 500 | 2.032 | 1.976 | 2.004 | 1.813 | | | 250 | 1.658 | 1.543 | 1.601 | 1.410 | | | 125 | 1.225 | 1.202 | 1.214 | 1.023 | | | 62.5 | 0.869 | 0.824 | 0.847 | 0.656 | | | 31.2 | 0.431 | 0.407 | 0.419 | 0.228 | | | 15.6 | 0.341 | 0.315 | 0.328 | 0.137 | | | 0 | 0.194 | 0.188 | 0.191 | | |
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货期
3-5 working days