产品描述
The Rat Agrin (AGRN) ELISA kit is a reliable and accurate tool for measuring Agrin protein levels in samples from Rattus norvegicus (Rat). Agrin, encoded by the AGRN gene, is a protein that plays a crucial role in the development and maintenance of the neuromuscular junction.
This ELISA kit provides a simple and sensitive assay to quantify Rat Agrin in serum, plasma, and tissue homogenates with a detection range of 78 pg/mL to 5000 pg/mL and a sensitivity of 19.5 pg/mL. The assay can be completed in just 1-5 hours, making it an efficient and convenient tool for research purposes.
The Rat Agrin (AGRN) ELISA kit requires a sample volume of only 50-100 µl, minimizing the need for large sample sizes. Detection is performed at a wavelength of 450 nm, with results displayed in a standard curve format.
别名
Agrn ELISA kit; AgrinAgrin [Cleaved into: Agrin N-terminal 110 kDa subunit; Agrin C-terminal 110 kDa subunit; Agrin C-terminal 90 kDa fragment ELISA kit; C90); Agrin C-terminal 22 kDa fragment ELISA kit; C22)] ELISA kit
缩写
AGRN
Uniprot No
P25304
灵敏度
19.5 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat AGRN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
90-96 |
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| 1:2 |
Average % |
84 |
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| Range % |
80-88 |
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| 1:4 |
Average % |
83 |
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| Range % |
80-86 |
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| 1:8 |
Average % |
89 |
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| Range % |
86-91 |
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回收率
| The recovery of rat AGRN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
100 |
97-103 |
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| EDTA plasma (n=4) |
96 |
93-99 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 5000 |
2.177 |
2.198 |
2.188 |
2.091 |
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| 2500 |
1.847 |
1.863 |
1.855 |
1.758 |
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| 1250 |
1.293 |
1.273 |
1.283 |
1.186 |
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| 625 |
0.811 |
0.846 |
0.829 |
0.732 |
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| 312.5 |
0.505 |
0.496 |
0.501 |
0.404 |
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| 156 |
0.301 |
0.321 |
0.311 |
0.214 |
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| 78 |
0.188 |
0.189 |
0.189 |
0.092 |
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| 0 |
0.096 |
0.098 |
0.097 |
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货期
3-5 working days
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