产品描述

This Human ACACA ELISA Kit was designed for the quantitative measurement of Human ACACA protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 9.38 pg/mL-600 pg/mL and the sensitivity is 2.34 pg/mL.

别名

ACAC ELISA Kit; ACACA ELISA Kit; ACACA_HUMAN ELISA Kit; ACACB ELISA Kit; ACC alpha ELISA Kit; ACC ELISA Kit; ACC beta ELISA Kit; ACC-alpha ELISA Kit; ACC1 ELISA Kit; ACC2 ELISA Kit; ACCA ELISA Kit; ACCB ELISA Kit; Acetyl CoA carboxylase 1 ELISA Kit; Acetyl CoA carboxylase 2 ELISA Kit; Acetyl CoA carboxylase alpha ELISA Kit; Acetyl CoA carboxylase beta ELISA Kit; Acetyl Coenzyme A carboxylase alpha ELISA Kit; Acetyl Coenzyme A carboxylase beta ELISA Kit; Biotin carboxylase ELISA Kit; COA1 ELISA Kit; COA2 ELISA Kit; HACC275 ELISA Kit; OTTHUMP00000164069 ELISA Kit; OTTHUMP00000164070 ELISA Kit; OTTHUMP00000164076 ELISA Kit; OTTHUMP00000240532 ELISA Kit

缩写

ACACA

Uniprot No

Q13085

灵敏度

2.34 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ACACA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:5		Average %		98
		Range %		96-102
1:10		Average %		107
		Range %		103-114
1:20		Average %		94
		Range %		90-98
1:40		Average %		88
		Range %		86-95

回收率

The recovery of human ACACA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		105		99-108
EDTA plasma (n=4)		94		90-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected     600 2.169 2.202 2.186 2.004     300 1.702 1.814 1.758 1.576     150 1.202 1.254 1.228 1.046     75 0.711 0.747 0.729 0.547     37.5 0.503 0.506 0.505 0.323     18.75 0.284 0.298 0.291 0.109     9.38 0.236 0.249 0.243 0.061     0 0.174 0.189 0.182 　         .

货期

3-5 working days