产品描述

The Human Caspase 1 (Casp-1) ELISA Kit is suitable for quantitative detection of Casp-1 in serum, plasma, and tissue homogenates. Samples or standards are added into the microwells and bind to the anti-human Casp-1 antibody immobilized on the microplate. A sandwich is formed by the addition of the biotin-conjugated Casp-1 antibody. The TMB substrate solution is added that reacts with the enzyme-antibody-target complex to elicit a chromogenic reaction. The intensity of the solution color is directly proportional to the concentration of Casp-1 present in the original specimen. This kit has been verified with a wide detection range, high sensitivity, excellent specificity, precision less than 10%, good linearity, high recovery, and good consistency between lots. Refer to the product instructions for more verification details.

Casp-1 is synthesized as an inactive zymogen that is recruited and activated by inflammasomes. It plays a crucial role in cell immunity as an inflammatory response inhibitor. After activation by inflammasomes, Casp-1 initiates a pro-inflammatory response through the cleavage and subsequent activation of the two inflammatory cytokines, IL-1β and IL-18 as well as gasdermin D (GSDMD)-mediated pyroptosis, thus killing the infectious agent. In the absence of GSDMD, Casp-1 induces apoptosis involves the Bid-caspase-9-caspase-3 axis, which can elicit secondary necrosis/pyroptosis mediated by GSDME/DFNA5.

别名

CASP-1 ELISA Kit; CASP1 ELISA Kit; CASP1_HUMAN ELISA Kit; Caspase 1 ELISA Kit; Caspase-1 subunit p10 ELISA Kit; ICE ELISA Kit; IL-1 beta-converting enzyme ELISA Kit; IL-1BC ELISA Kit; IL1 beta converting enzyme ELISA Kit; IL1B convertase ELISA Kit; Interleukin 1 beta convertase ELISA Kit; Interleukin 1B converting enzyme ELISA Kit; Interleukin-1 beta convertase ELISA Kit; Interleukin-1 beta-converting enzyme ELISA Kit; p45 ELISA Kit

缩写

CASP1

Uniprot No

P29466

灵敏度

0.39 ng/ml

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

货期

3-5 working days