产品描述
This Human CD2AP ELISA Kit was designed for the quantitative measurement of Human CD2AP protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.7 pg/mL.
别名
Adapter protein CMS ELISA Kit; AL024079 ELISA Kit; C78928 ELISA Kit; Cas ligand with multiple SH3 domains ELISA Kit; CD2 associated protein ELISA Kit; CD2-associated protein ELISA Kit; CD2AP ELISA Kit; CD2AP_HUMAN ELISA Kit; CMS ELISA Kit; Mesenchyme to epithelium transition protein with SH3 domains 1 ELISA Kit; METS 1 ELISA Kit; Mets1 ELISA Kit
缩写
CD2AP
Uniprot No
Q9Y5K6
灵敏度
4.7 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD2AP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 88 | | |
| Range % | 82-93 | | |
| 1:2 | Average % | 97 | | |
| Range % | 91-103 | | |
| 1:4 | Average % | 92 | | |
| Range % | 87-98 | | |
| 1:8 | Average % | 101 | | |
| Range % | 96-105 | | |
回收率
| The recovery of human CD2AP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
|
| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 89 | 82-94 | | |
| EDTA plasma (n=4) | 93 | 86-97 | | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | | 1200 | 2.864 | 2.755 | 2.810 | 2.694 | | | | 600 | 2.137 | 2.212 | 2.175 | 2.059 | | | | 300 | 1.456 | 1.537 | 1.497 | 1.381 | | | | 150 | 0.956 | 0.946 | 0.951 | 0.835 | | | | 75 | 0.497 | 0.489 | 0.493 | 0.377 | | | | 37.5 | 0.274 | 0.289 | 0.282 | 0.166 | | | | 18.75 | 0.218 | 0.210 | 0.214 | 0.098 | | | | 0 | 0.116 | 0.115 | 0.116 | | | |
|
货期
3-5 working days