产品描述
This Human Coll2-1 ELISA Kit was designed for the quantitative measurement of Human Coll2-1 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 47 pg/mL-3000 pg/mL and the sensitivity is 11.72 pg/mL.
缩写
Coll2-1
灵敏度
11.75 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human Coll2-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:100 | Average % | 85 | |
| Range % | 80-90 | |
| 1:200 | Average % | 98 | |
| Range % | 90-105 | |
| 1:400 | Average % | 95 | |
| Range % | 90-100 | |
| 1:800 | Average % | 92 | |
| Range % | 87-97 | |
回收率
| The recovery of human Coll2-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
89 |
83-95 |
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| EDTA plasma (n=4) |
98 |
92-105 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 3000 |
2.513 |
2.487 |
2.500 |
2.417 |
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| 1500 |
1.847 |
1.794 |
1.821 |
1.738 |
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| 750 |
1.198 |
1.112 |
1.155 |
1.072 |
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| 375 |
0.754 |
0.709 |
0.732 |
0.649 |
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| 187.5 |
0.351 |
0.337 |
0.344 |
0.261 |
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| 94 |
0.198 |
0.183 |
0.191 |
0.108 |
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| 47 |
0.134 |
0.125 |
0.130 |
0.047 |
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| 0 |
0.084 |
0.081 |
0.083 |
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货期
3-5 working days