产品描述
The human BAX ELISA kit (CSB-E09344h) is designed for the quantitative measurement of human BAX protein in serum, plasma, or tissue homogenates. It quantitates human BAX with 0.312 ng/ml sensitivity and shows excellent specificity for human BAX. It uses the bi-antibody sandwich enzyme immunoassay technique. This assay employs a biotin-conjugated BAX antibody that recognizes the analyte bound by the immobilized BAX antibody, forming an antibody-analyte-antibody complex. The immune complex is further detected by avidin-conjugated HRP. The TMB solution is added into the wells and turns blue and finally turns yellow after the addition of the stop solution. Solution color develops in proportion to the amount of BAX in the sample. The O.D. value is measured using a microplate reader at 450 nm and is used to determine the concentration of the human BAX in the sample.
BAX is one of the pro-apoptotic proteins of the BCL2 gene family that mediates mitochondrial fusion. BAX is essential for mitochondrial fusion in healthy cells. During apoptosis, BAX is recruited and oligomerized at mitochondrial foci. BAX is involved in the formation of pores that enables the mitochondrion to cytochrome c and subsequent activation of caspase cascade release, finally leading to apoptosis.
别名
Apoptosis regulator BAX ELISA Kit; BAX ELISA Kit; Bax-protein ELISA Kit; BAX_HUMAN ELISA Kit; BAXA ELISA Kit; Baxdelta2G9 ELISA Kit; Baxdelta2G9omega ELISA Kit; Baxdelta2omega ELISA Kit; Bcl-2-like protein 4 ELISA Kit; BCL2 associated X protein ELISA Kit; BCL2 associated X protein omega ELISA Kit; BCL2 associated X protein transcript variant delta2 ELISA Kit; Bcl2-L-4 ELISA Kit; BCL2L4 ELISA Kit; membrane isoform alpha ELISA Kit
缩写
BAX
Uniprot No
Q07812
灵敏度
0.312 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human BAX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
87-101 |
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| 1:2 |
Average % |
94 |
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| Range % |
91-103 |
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| 1:4 |
Average % |
95 |
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| Range % |
92-100 |
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| 1:8 |
Average % |
94 |
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| Range % |
88-97 |
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回收率
| The recovery of human BAX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
102 |
96-109 |
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| EDTA plasma (n=4) |
96 |
90-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 80 |
2.305 |
2.215 |
2.260 |
2.108 |
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| 40 |
1.675 |
1.765 |
1.720 |
1.568 |
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| 20 |
1.164 |
1.172 |
1.168 |
1.016 |
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| 10 |
0.795 |
0.809 |
0.802 |
0.650 |
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| 5 |
0.453 |
0.447 |
0.450 |
0.298 |
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| 2.5 |
0.322 |
0.336 |
0.329 |
0.177 |
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| 1.25 |
0.225 |
0.219 |
0.222 |
0.070 |
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| 0 |
0.152 |
0.151 |
0.152 |
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货期
3-5 working days
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