产品描述
This Mouse acetyl-CoA ELISA Kit was designed for the quantitative measurement of Mouse acetyl-CoA protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL.
缩写
acetyl-CoA
灵敏度
0.195 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Acetyl-CoA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:20 |
Average % |
91 |
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| Range % |
87-93 |
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| 1:40 |
Average % |
103 |
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| Range % |
100-105 |
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| 1:80 |
Average % |
93 |
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| Range % |
87-96 |
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| 1:160 |
Average % |
98 |
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| Range % |
93-101 |
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回收率
| The recovery of mouse Acetyl-CoA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
89-97 |
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| EDTA plasma (n=4) |
96 |
92-98 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 50 |
2.192 |
2.114 |
2.153 |
1.996 |
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| 25 |
1.632 |
1.647 |
1.640 |
1.483 |
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| 12.5 |
1.124 |
1.110 |
1.117 |
0.960 |
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| 6.25 |
0.713 |
0.688 |
0.701 |
0.544 |
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| 3.12 |
0.404 |
0.421 |
0.413 |
0.256 |
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| 1.56 |
0.291 |
0.278 |
0.285 |
0.128 |
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| 0.78 |
0.220 |
0.212 |
0.216 |
0.059 |
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| 0 |
0.158 |
0.156 |
0.157 |
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货期
3-5 working days