产品描述
This Human CFL1 ELISA Kit was designed for the quantitative measurement of Human CFL1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.
别名
18 kDa phosphoprotein ELISA Kit; CFL 1 ELISA Kit; CFL ELISA Kit; CFL1 ELISA Kit; COF1_HUMAN ELISA Kit; Cofilin 1 ELISA Kit; Cofilin 1 non muscle ELISA Kit; Cofilin ELISA Kit; Cofilin non muscle isoform ELISA Kit; Cofilin-1 ELISA Kit; epididymis secretory protein Li 15 ELISA Kit; HEL-S-15 ELISA Kit; non-muscle isoform ELISA Kit; p18 ELISA Kit
缩写
CFL1
Uniprot No
P23528
灵敏度
6.25 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human CFL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 89 | | |
| Range % | 85-93 | | |
| 1:2 | Average % | 102 | | |
| Range % | 98-105 | | |
| 1:4 | Average % | 97 | | |
| Range % | 95-00 | | |
| 1:8 | Average % | 88 | | |
| Range % | 84-92 | | |
回收率
| The recovery of human CFL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 93 | 90-96 | | |
| EDTA plasma (n=4) | 105 | 101-109 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | | 1600 | 2.604 | 2.638 | 2.621 | 2.486 | | | | 800 | 2.078 | 2.041 | 2.060 | 1.925 | | | | 400 | 1.329 | 1.305 | 1.317 | 1.182 | | | | 200 | 0.804 | 0.797 | 0.801 | 0.666 | | | | 100 | 0.431 | 0.456 | 0.444 | 0.309 | | | | 50 | 0.294 | 0.287 | 0.291 | 0.156 | | | | 25 | 0.213 | 0.206 | 0.210 | 0.075 | | | | 0 | 0.136 | 0.134 | 0.135 | | | |
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货期
3-5 working days