产品描述

This Human BCL2L11 ELISA Kit was designed for the quantitative measurement of Human BCL2L11 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.44 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL .

别名

BCL2 like 11 ELISA Kit; B2L11_HUMAN ELISA Kit; BAM ELISA Kit; Bcl 2 interacting protein Bim ELISA Kit; Bcl 2 related ovarian death agonist ELISA Kit; Bcl-2-like protein 11 ELISA Kit; BCL2 interacting mediator of cell death ELISA Kit; BCL2 like 11 (apoptosis facilitator) ELISA Kit; BCL2 like protein 11 ELISA Kit; Bcl2-interacting mediator of cell death ELISA Kit; Bcl2-L-11 ELISA Kit; Bcl2l11 ELISA Kit; BIM alpha6 ELISA Kit; BIM ELISA Kit; BIM beta6 ELISA Kit; BIM beta7 ELISA Kit; BimEL ELISA Kit; BimL ELISA Kit; BOD ELISA Kit

缩写

BCL2L11

Uniprot No

O43521

灵敏度

5.86 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human BCL2L11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		96
		Range %		90-102
1:2		Average %		92
		Range %		84-97
1:4		Average %		99
		Range %		95-105
1:8		Average %		97
		Range %		92-104

回收率

The recovery of human BCL2L11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		96		90-102
EDTA plasma (n=4)		102		98-108

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1500 2.194 2.187 2.191 1.991 750 1.506 1.537 1.522 1.322 375 1.012 1.036 1.024 0.824 187.5 0.659 0.689 0.674 0.474 93.75 0.445 0.426 0.436 0.236 46.88 0.345 0.324 0.335 0.135 23.44 0.260 0.282 0.271 0.071 0 0.201 0.198 0.200

货期

3-5 working days