产品描述

This Human AKR1B1 ELISA Kit was designed for the quantitative measurement of Human AKR1B1 protein in serum, plasma, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

别名

ADR ELISA Kit; AKR1B 1 ELISA Kit; Akr1b1 ELISA Kit; Aldehyde reductase 1 ELISA Kit; Aldehyde reductase ELISA Kit; Aldo keto reductase family 1; member B1 ELISA Kit; Aldo-keto reductase family 1 member B1 ELISA Kit; aldo-keto reductase family 1; member B1 (aldose reductase) ELISA Kit; Aldose reductase ELISA Kit; aldr 1 ELISA Kit; ALDR_HUMAN ELISA Kit; aldr1 ELISA Kit; ALR2 ELISA Kit; AR ELISA Kit; Lii5 2 CTCL tumor antigen ELISA Kit; Low Km aldose reductase ELISA Kit; MGC1804 ELISA Kit

缩写

AKR1B1

Uniprot No

P15121

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human AR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		86
		Range %		82-90
1:2		Average %		94
		Range %		90-98
1:4		Average %		87
		Range %		83-90
1:8		Average %		100
		Range %		95-105

回收率

The recovery of human AR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		98		94-102
EDTA plasma (n=4)		92		87-96

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 20 2.392 2.375 2.384 2.222 10 1.859 1.804 1.832 1.670 5 1.411 1.435 1.423 1.261 2.5 0.966 0.968 0.967 0.805 1.25 0.540 0.568 0.554 0.392 0.625 0.363 0.368 0.366 0.204 0.312 0.235 0.241 0.238 0.076 0 0.164 0.160 0.162

货期

3-5 working days